Difference between revisions of "Part:BBa K3409010"

 
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Contains the elements necessary to express the three first genes from the Microcin PDI gene cluster (mcpM: coding for microcin PDI, mcpI: coding for the immunity protein, mcpA: coding for the activator protein). All three genes are under the control of the same promoter and the sequence is organized as follows: a promoter (BBa_J23111), an RBS (BBa_J61127), the CDS for McpM-His (BBa_K3409003), an RBS (BBa_J61130), the CDS for McpI (BBa_K3409004), an RBS (BBa_J61118), the CDS for McpA (BBa_K3409005) and a double forward terminator (BBa_B0015). Note that it must be expressed with the two other genes from the cluster (mcpD and mcpB) for secretion of McpM. Will constitutively generate McpM, McpI and McpA.  
 
Contains the elements necessary to express the three first genes from the Microcin PDI gene cluster (mcpM: coding for microcin PDI, mcpI: coding for the immunity protein, mcpA: coding for the activator protein). All three genes are under the control of the same promoter and the sequence is organized as follows: a promoter (BBa_J23111), an RBS (BBa_J61127), the CDS for McpM-His (BBa_K3409003), an RBS (BBa_J61130), the CDS for McpI (BBa_K3409004), an RBS (BBa_J61118), the CDS for McpA (BBa_K3409005) and a double forward terminator (BBa_B0015). Note that it must be expressed with the two other genes from the cluster (mcpD and mcpB) for secretion of McpM. Will constitutively generate McpM, McpI and McpA.  
  
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===Usage===
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===Usage and Biology===
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This part is used to produce Microcin PDI, its immunity protein for the producing strain as well as the activator protein. For microcin PDI to be effective against a target strain it must be excreted.
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===Biology===
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Attention must be given to the quantity of McpM and McpI produced as there must be a balance between both. Without the immunity protein, the producer strain will die.
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===Characterization===
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Revision as of 20:21, 25 October 2020


Microcin PDI cluster genes for McpM, McpI, McpA

Contains the elements necessary to express the three first genes from the Microcin PDI gene cluster (mcpM: coding for microcin PDI, mcpI: coding for the immunity protein, mcpA: coding for the activator protein). All three genes are under the control of the same promoter and the sequence is organized as follows: a promoter (BBa_J23111), an RBS (BBa_J61127), the CDS for McpM-His (BBa_K3409003), an RBS (BBa_J61130), the CDS for McpI (BBa_K3409004), an RBS (BBa_J61118), the CDS for McpA (BBa_K3409005) and a double forward terminator (BBa_B0015). Note that it must be expressed with the two other genes from the cluster (mcpD and mcpB) for secretion of McpM. Will constitutively generate McpM, McpI and McpA.

Usage

This part is used to produce Microcin PDI, its immunity protein for the producing strain as well as the activator protein. For microcin PDI to be effective against a target strain it must be excreted.

Biology

Attention must be given to the quantity of McpM and McpI produced as there must be a balance between both. Without the immunity protein, the producer strain will die.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]