Difference between revisions of "Part:BBa K3440019"

Revision as of 20:21, 25 October 2020

MtrB under Lux promoter

Pconst(BBa_J23100) - RBS(BBa_B0034) - LuxR(BBa_C0062) - DT(BBa_B0015) - Plux(BBa_R0062) - RBS(BBa_B0034) - mtrb(BBa_K3146001) - DT(BBa_B0015)

Usage and Biology

This is an intermediate part of the oscillatory system which can be used to build our final modular biosensor BBa_K3440021. LuxR which is originally from Vibrio Fischeri, is constitutively expressed under the J23100 promoter. In the presence of 3OC6-HSL (a lactone produced by LuxI from Vibrio Fischeri), the LuxR:3OC6-HSL complex can activate Plux, which controls production of MtrB. This oscillatory part will be present in Shewanella oneidensis MR-1 mtrb knockout.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 985

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3440019 short</partinfo>
-Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxR(BBa_C0062)-DT(BBa_B0015)-Plux(BBa_R0062)-RBS(BBa_B0034)-mtrb(BBa_K3146001)-DT(BBa_B0015)
+Pconst(BBa_J23100) - RBS(BBa_B0034) - LuxR(BBa_C0062) - DT(BBa_B0015) - Plux(BBa_R0062) - RBS(BBa_B0034) - mtrb(BBa_K3146001) - DT(BBa_B0015)
 ===Usage and Biology===