Difference between revisions of "Part:BBa K3429000"
Line 4: | Line 4: | ||
− | IPTG-inducible promoter as used in Huang et al. for TasA fusion protein expression.<sup> [1] </sup> We decided to use the same promoter and terminator system as Huang et al. did for | + | IPTG-inducible promoter as used in Huang et al. for TasA fusion protein expression.<sup> [1] </sup> We decided to use the same promoter and terminator system as Huang et al. did for their TasA-sfGFP as well as our TasA-CotA/CuO/EreB fusion proteins. |
Revision as of 18:57, 25 October 2020
Pgrac promoter
IPTG-inducible promoter as used in Huang et al. for TasA fusion protein expression. [1] We decided to use the same promoter and terminator system as Huang et al. did for their TasA-sfGFP as well as our TasA-CotA/CuO/EreB fusion proteins.
References
[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]