Difference between revisions of "Part:BBa K3467003:Experience"

 
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In our project, we designed three basic part, targeting to three different genes of locust. In our design, we selected Locust migratoria gene trypsin-like serine protease, chitin synthase and ATPase subunitH gene as our target gene. By using specific program to design dsRNA sequence, we finally designed our three basic parts: TSP dsRNA sequence, CHS dsRNA sequence and ATPase dsRNA sequence.
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__NOTOC__
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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We also measured their effect to locust by injection experiment.
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how you used this part and how it worked out.
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As the result shows:
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===Applications of BBa_K3467003===
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Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days.  
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===User Reviews===
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<!-- DON'T DELETE --><partinfo>BBa_K3467003 StartReviews</partinfo>
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Here is the survival rate that we measure during the whole experiment process.
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<!-- Template for a user review
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<partinfo>BBa_K3467003 AddReview number</partinfo>
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<I>Username</I>
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Enter the review inofrmation here.
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<!-- DON'T DELETE --><partinfo>BBa_K3467003 EndReviews</partinfo>
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Revision as of 18:17, 25 October 2020

In our project, we designed three basic part, targeting to three different genes of locust. In our design, we selected Locust migratoria gene trypsin-like serine protease, chitin synthase and ATPase subunitH gene as our target gene. By using specific program to design dsRNA sequence, we finally designed our three basic parts: TSP dsRNA sequence, CHS dsRNA sequence and ATPase dsRNA sequence.

We also measured their effect to locust by injection experiment.

As the result shows:

Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days.

Here is the survival rate that we measure during the whole experiment process.