Difference between revisions of "Part:BBa K3589210"

Revision as of 17:46, 25 October 2020

Level 2 � spectinomycin resistance + cCA_marLac_SP20-HA-RGS-8His

This composite part contains a spectinomycin resistance (BBa_K3002102) and the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) fused with a SP20-HA-RGS-8His-tag (BBa_K3589150) for an efficient secretion, easy detection via HA-antibody and purification via nickel-NTA-column.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2401
Illegal PstI site found at 3795
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2401
Illegal NheI site found at 2665
Illegal PstI site found at 3795
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2401
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2401
Illegal PstI site found at 3795
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2401
Illegal PstI site found at 3795
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3655
Illegal NgoMIV site found at 4374
Illegal NgoMIV site found at 4825
Illegal NgoMIV site found at 5471
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3589210 short</partinfo>
-XXXXX
+<html>
+<p>
+This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the wildtype laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>) fused with a SP20-HA-RGS-8His-tag (<a href="https://parts.igem.org/Part:BBa_K3589150">BBa_K3589150</a>) for an efficient secretion, easy detection via HA-antibody and purification via nickel-NTA-column.
+</p>
+</html>
 <!-- Add more about the biology of this part here