Difference between revisions of "Part:BBa K3589209"

 
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the mutant laccase from <I>Botrytis aclada</I> (<a href="https://parts.igem.org/Part:BBa_K3589108">BBa_K3589108</a>) fused with an SP20-HA-RGS-8His-tag (<a href="https://parts.igem.org/Part:BBa_K3589150">BBa_K3589150</a>) for an efficient secretion, easy detection via HA-antibody and purification via nickel-NTA-column.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:44, 25 October 2020


Level 2 spectinomycin resistance + cCA_BaLac_SP20-HA-RGS-8His

This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant laccase from Botrytis aclada (BBa_K3589108) fused with an SP20-HA-RGS-8His-tag (BBa_K3589150) for an efficient secretion, easy detection via HA-antibody and purification via nickel-NTA-column.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3586
    Illegal PstI site found at 3673
    Illegal PstI site found at 3737
    Illegal PstI site found at 4109
    Illegal PstI site found at 4397
    Illegal PstI site found at 4474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3586
    Illegal PstI site found at 3673
    Illegal PstI site found at 3737
    Illegal PstI site found at 4109
    Illegal PstI site found at 4397
    Illegal PstI site found at 4474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal BglII site found at 4033
    Illegal BglII site found at 4992
    Illegal BamHI site found at 4372
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3586
    Illegal PstI site found at 3673
    Illegal PstI site found at 3737
    Illegal PstI site found at 4109
    Illegal PstI site found at 4397
    Illegal PstI site found at 4474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3586
    Illegal PstI site found at 3673
    Illegal PstI site found at 3737
    Illegal PstI site found at 4109
    Illegal PstI site found at 4397
    Illegal PstI site found at 4474
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 6232
  • 1000
    COMPATIBLE WITH RFC[1000]