Difference between revisions of "Part:BBa K3589208"

 
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the wildtype laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>) fused with an 3xHA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) for easy detection via HA-antibody.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:38, 25 October 2020


Level 2 spectinomycin resistance + marLac_3xHA

This composite part contains a spectinomycin resistance (BBa_K3002102) and the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) fused with an 3xHA-tag (BBa_K3002017) for easy detection via HA-antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3728
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3728
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3728
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3728
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3588
    Illegal NgoMIV site found at 4307
    Illegal NgoMIV site found at 4758
    Illegal NgoMIV site found at 5311
  • 1000
    COMPATIBLE WITH RFC[1000]