Difference between revisions of "Part:BBa K3589207"
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the mutant laccase from <I>Botrytis aclada</I> (<a href="https://parts.igem.org/Part:BBa_K3589108">BBa_K3589108</a>) fused with an 3xHA-tag (<a href="https://parts.igem.org/Part: BBa_K3002017">BBa_K3002017</a>) for easy detection via HA-antibody.
 
This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the mutant laccase from <I>Botrytis aclada</I> (<a href="https://parts.igem.org/Part:BBa_K3589108">BBa_K3589108</a>) fused with an 3xHA-tag (<a href="https://parts.igem.org/Part: BBa_K3002017">BBa_K3002017</a>) for easy detection via HA-antibody.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:35, 25 October 2020


L2 spectinomycin resistance + BaLac_3xHA

This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant laccase from Botrytis aclada (BBa_K3589108) fused with an 3xHA-tag (BBa_K3002017) for easy detection via HA-antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3519
    Illegal PstI site found at 3606
    Illegal PstI site found at 3670
    Illegal PstI site found at 4042
    Illegal PstI site found at 4330
    Illegal PstI site found at 4407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3519
    Illegal PstI site found at 3606
    Illegal PstI site found at 3670
    Illegal PstI site found at 4042
    Illegal PstI site found at 4330
    Illegal PstI site found at 4407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal BglII site found at 3966
    Illegal BglII site found at 4925
    Illegal BamHI site found at 4305
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3519
    Illegal PstI site found at 3606
    Illegal PstI site found at 3670
    Illegal PstI site found at 4042
    Illegal PstI site found at 4330
    Illegal PstI site found at 4407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3519
    Illegal PstI site found at 3606
    Illegal PstI site found at 3670
    Illegal PstI site found at 4042
    Illegal PstI site found at 4330
    Illegal PstI site found at 4407
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 6046
  • 1000
    COMPATIBLE WITH RFC[1000]