Difference between revisions of "Part:BBa K3657023"
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<p>It was proved by Kunkler et al. that this sequence creates a triple helix with the RNA part (<a href= "https://parts.igem.org/Part:BBa_K3657022">BBa_K3657022</a>) in vitro.</p> | <p>It was proved by Kunkler et al. that this sequence creates a triple helix with the RNA part (<a href= "https://parts.igem.org/Part:BBa_K3657022">BBa_K3657022</a>) in vitro.</p> | ||
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<partinfo>BBa_K3657023 parameters</partinfo> | <partinfo>BBa_K3657023 parameters</partinfo> | ||
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Revision as of 17:10, 25 October 2020
DNA Part of an RNA·DNA-DNA triple helix
It was proved by Kunkler et al. that this sequence creates a triple helix with the RNA part (BBa_K3657022) in vitro.
More information available here: LINK
Usage and Biology
Triple helices are one of the alternative structures formed by nucleic acids. In addition to the Watson-Crick interactions between base pairs, Hoogsteen interactions are crucial for the formation of the triple helix. The binding is sequence specific.
Part Design
You can generate analogical parts yourself as following:
- Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.
- Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Kunkler et al.
References
Kunkler CN, Hulewicz JP, Hickman SC, Wang MC, McCown PJ, Brown JA. Stability of an RNA•DNA-DNA triple helix depends on base triplet composition and length of the RNA third strand. Nucleic Acids Res. 2019; 47(14):7213-7222.