Difference between revisions of "Part:BBa K3589202"
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This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), a 3xHA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) and the coding sequence of the mutant laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>) plus the MoClo connectors for positions B2-B3 ( <a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 ( <a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>). | This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), a 3xHA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) and the coding sequence of the mutant laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>) plus the MoClo connectors for positions B2-B3 ( <a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 ( <a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>). | ||
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Revision as of 17:09, 25 October 2020
L1 Wildtype marLac + HA (Phytobrick)
This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), a 3xHA-tag (BBa_K3002017) and the coding sequence of the mutant laccase from an uncultured marine bacteria (BBa_K3589109) plus the MoClo connectors for positions B2-B3 ( BBa_K3002303), B4-B5 ( BBa_K3002304) and B5-B6 (BBa_K3002305).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1312
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal PstI site found at 1312 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1312
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1312
Illegal NgoMIV site found at 1172
Illegal NgoMIV site found at 1891
Illegal NgoMIV site found at 2342
Illegal NgoMIV site found at 2895 - 1000COMPATIBLE WITH RFC[1000]