Difference between revisions of "Part:BBa K3696004"

Revision as of 16:59, 25 October 2020

A DNA sequence that contains T7 promoter and can be transcribed to Spinach RNA, which can react with

BBa_K3696004--BBa_K3696007 are used in TO-DNA testings, the protocol is shown below:

TO-DNA protocol

TO-DNA system protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

Key strand concentration testing

From the detected fluorescence, we determine that Key1 OR-IN1 is more effective than Key1 OR-IN2, and its limit for detection is 1 nM.

Key strand 1 complementary sequence testing

However, we find that the reaction might start earlier than expected after combining TO-DNA system to DNAzyme, so we decide to give up this system.

Sequence and Features

Assembly Compatibility:

@@ Line 5: / Line 5: @@
 <b>BBa_K3696004--BBa_K3696007</b> are used in TO-DNA testings, the protocol is shown below:<br>
 [[File:T--OSA--protocol003.1.png|300px|thumb|center|TO-DNA protocol]]<br>
-[[File:T--OSA--protocol003.2.png|300px|thumb|center|TO-DNA system protocol]]<br>
+[[File:T--OSA--protocol003.2.png|300px|thumb|center|TO-DNA system protocol]]
 <br>
 The system is put into the enzyme marker and reacted for 60 minutes at 37℃.<br>