Difference between revisions of "Part:BBa K3696004"

Revision as of 16:55, 25 October 2020

A DNA sequence that contains T7 promoter and can be transcribed to Spinach RNA, which can react with

BBa_K3696004--BBa_K3696007 are used in TO-DNA testings, the protocol is shown below:

TO-DNA protocol

TO-DNA system protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

Key strand concentration testing

From the detected fluorescence, we determine that Key1 OR-IN1 is more effective than Key1 OR-IN2, and its limit for detection is 1 nM.

Key strand 1 complementary sequence testing

However, we find that the reaction might start earlier than expected after combining TO-DNA system to DNAzyme, so we decide to give up this system.

Sequence and Features

Assembly Compatibility:

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 <br>
 [[File:T--OSA--parts003.1.png|450px|thumb|center|Key strand concentration testing]]<br>
-<br>
 From the detected fluorescence, we determine that Key1 OR-IN1 is more effective than Key1 OR-IN2, and its limit for detection is 1 nM.<br>
 <br>
 [[File:T--OSA--parts003.2.png|450px|thumb|center|Key strand 1 complementary sequence testing]]<br>
-<br>
 However, we find that the reaction might start earlier than expected after combining TO-DNA system to DNAzyme, so we decide to give up this system.<br>
 <br>