Difference between revisions of "Part:BBa K3696003"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K3696003 short</partinfo>
 
<partinfo>BBa_K3696003 short</partinfo>
  
<b>BBa_K3696000, BBa_K3696002--BBa_K3696003</b> are used in HTDC testings, the protocol is shown below:
+
<b>BBa_K3696000, BBa_K3696002--BBa_K3696003</b> are used in HTDC testings, the protocol is shown below:<br>
 
+
[[File:T--OSA--protocol001.png|300px|thumb|center|HTDC protocol]]<br>
[[File:T--OSA--protocol001.png|300px|thumb|center|HTDC protocol]]
+
<br>
 
+
The system is put into the enzyme marker and reacted for 60 minutes at 37℃.<br>
The system is put into the enzyme marker and reacted for 60 minutes at 37&#8451;.
+
[[File:T--OSA--part001.png|450px|thumb|center|HTDC Pb2+ testing]]<br>
 
+
From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.<br>
[[File:T--OSA--part001.png|450px|thumb|center|HTDC Pb2+ testing]]
+
<br>
 
+
From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.
+
 
+
  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
===Usage and Biology===
+
===Usage and Biology===-->
  
 
<!-- -->
 
<!-- -->

Latest revision as of 16:06, 25 October 2020


A short DNA sequence that can bind to 8-17 DNAzyme and 8-17 substrate strand.

BBa_K3696000, BBa_K3696002--BBa_K3696003 are used in HTDC testings, the protocol is shown below:

HTDC protocol


The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

HTDC Pb2+ testing

From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]