Difference between revisions of "Part:BBa K3482000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | RNA thermosensors are non-coding RNA molecules that are thermosensitive. When placed upstream of a gene, they can regulate the translation of this gene. RNA thermosensors usually act by changing in the secondary structure due to the change of temperature. These changes will block or free the access of the ribosome to the ribosome binding site (RBS) and thus regulate the translation. Thermosensors are often | + | RNA thermosensors are non-coding RNA molecules that are thermosensitive. When placed upstream of a gene, they can regulate the translation of this gene. RNA thermosensors usually act by changing in the secondary structure due to the change of temperature. These changes will block or free the access of the ribosome to the ribosome binding site (RBS) and thus regulate the translation. Thermosensors are often involved in the regulation of heat-shock and cold-shock proteins. |
We used this RNA thermosensors to regulate the expression of a toxin or an antitoxin in our kill-switch | We used this RNA thermosensors to regulate the expression of a toxin or an antitoxin in our kill-switch | ||
Revision as of 15:50, 25 October 2020
heat-inducible RNA-thermosensor 2U
The 2U RNA thermosensor is inserted directly upstream of the gene of interest. The gene expression is then modulated by temperature.
Usage and Biology
RNA thermosensors are non-coding RNA molecules that are thermosensitive. When placed upstream of a gene, they can regulate the translation of this gene. RNA thermosensors usually act by changing in the secondary structure due to the change of temperature. These changes will block or free the access of the ribosome to the ribosome binding site (RBS) and thus regulate the translation. Thermosensors are often involved in the regulation of heat-shock and cold-shock proteins.
We used this RNA thermosensors to regulate the expression of a toxin or an antitoxin in our kill-switch
Characterization
All the data concerning the characterization of that part can be found in the composite part : BBa_K3482017 .
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 14
Illegal PstI site found at 20 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 14
Illegal PstI site found at 20 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 14
Illegal PstI site found at 20 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 14
Illegal PstI site found at 20 - 1000COMPATIBLE WITH RFC[1000]