Difference between revisions of "Part:BBa K3503011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE). RFP is added due to the adhesion test. | |
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===Source=== | ===Source=== | ||
| − | + | The alpha-s1-casein is from the previous iGEM team, which was initiated based on Bos taurus; The mfp5 adhesion domain is from the previous iGEM team, which was initiated based on Mytilus galloprovincialisMytilus galloprovincialis. | |
===References=== | ===References=== | ||
Latest revision as of 15:19, 25 October 2020
mfp5-alpha casein-RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 727
Illegal BamHI site found at 1525 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2085
Illegal AgeI site found at 2197 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE). RFP is added due to the adhesion test.
Source
The alpha-s1-casein is from the previous iGEM team, which was initiated based on Bos taurus; The mfp5 adhesion domain is from the previous iGEM team, which was initiated based on Mytilus galloprovincialisMytilus galloprovincialis.