Difference between revisions of "Part:BBa K3696000"
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<b>BBa_K3696000, BBa_K3696002--BBa_K3696003</b> are used in HTDC testings, the protocol is shown below:<br> | <b>BBa_K3696000, BBa_K3696002--BBa_K3696003</b> are used in HTDC testings, the protocol is shown below:<br> | ||
| − | [[File:T--OSA--protocol001.png| | + | [[File:T--OSA--protocol001.png|300px|thumb|center|HTDC protocol]]<br> |
<br> | <br> | ||
The system is put into the enzyme marker and reacted for 60 minutes at 37℃.<br> | The system is put into the enzyme marker and reacted for 60 minutes at 37℃.<br> | ||
| − | [[File:T--OSA--part001.png| | + | [[File:T--OSA--part001.png|450px|thumb|center|HTDC Pb2+ testing]]<br> |
From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.<br> | From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.<br> | ||
Revision as of 14:58, 25 October 2020
A type of DNA oligonucleotide that can cleave towards its substrate after binding to target and Pb2+
BBa_K3696000, BBa_K3696002--BBa_K3696003 are used in HTDC testings, the protocol is shown below:
The system is put into the enzyme marker and reacted for 60 minutes at 37℃.
From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]