Difference between revisions of "Part:BBa K3503004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE). | |
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===Source=== | ===Source=== | ||
| − | + | The SR is from the previous iGEM team, which was initiated based on Homo sapiens; The cellulose-binding domain is from the previous iGEM team, which is from the Cellulosomal -scaffolding protein A (cipA) of Clostridium thermocellum including the endogenous linker sequences at the N and C-terminus. | |
===References=== | ===References=== | ||
Latest revision as of 14:56, 25 October 2020
CBD-SR-His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 146
Illegal BglII site found at 263
Illegal BglII site found at 332
Illegal BamHI site found at 107 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE).
Source
The SR is from the previous iGEM team, which was initiated based on Homo sapiens; The cellulose-binding domain is from the previous iGEM team, which is from the Cellulosomal -scaffolding protein A (cipA) of Clostridium thermocellum including the endogenous linker sequences at the N and C-terminus.