Difference between revisions of "Part:BBa K3696001"

Revision as of 14:50, 25 October 2020

A type of DNA oligonucleotide that can cleave towards its substrate after binding to Pb2+.

BBa_K3696001, BBa_K3696002 are used in 8-17 DNAzyme testings, the protocol is shown below:

8-17 DNAzyme protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃. From the detected fluorescence, we determine that:

8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.

8-17 DNAzyme(two parts) Pb2+ testing

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3696001 short</partinfo>
-<b>BBa_K3696001, BBa_K3696002</b> are used in 8-17 DNAzyme testings, the protocol is shown below: <br> [[File:T--OSA--protocol002.png|600px|thumb|center|8-17 DNAzyme protocol]] The system is put into the enzyme marker and reacted for 60 minutes at 37℃. From the detected fluorescence, we determine that: <br><br> 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.<br> [[File:T--OSA--parts002.png|600px|thumb|center|8-17 DNAzyme(two parts) Pb2+ testing]]
+<b>BBa_K3696001, BBa_K3696002</b> are used in 8-17 DNAzyme testings, the protocol is shown below: <br> [[File:T--OSA--protocol002.png|450px|thumb|center|8-17 DNAzyme protocol]] The system is put into the enzyme marker and reacted for 60 minutes at 37℃. From the detected fluorescence, we determine that: <br><br> 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.<br> [[File:T--OSA--parts002.png|450px|thumb|center|8-17 DNAzyme(two parts) Pb2+ testing]]
 <!-- Add more about the biology of this part here