Difference between revisions of "Part:BBa K3696001"
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<partinfo>BBa_K3696001 short</partinfo> | <partinfo>BBa_K3696001 short</partinfo> | ||
− | BBa_K3696001, BBa_K3696002 are used in 8-17 DNAzyme testings.The system is put into the enzyme marker and reacted for 60 minutes at | + | <b>BBa_K3696001, BBa_K3696002</b> are used in 8-17 DNAzyme testings, the protocol is shown below: <br> [[File:T--OSA--protocol002.png|600px|thumb|center|8-17 DNAzyme protocol]] The system is put into the enzyme marker and reacted for 60 minutes at 37℃. From the detected fluorescence, we determine that: <br><br> 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.<br> [[File:T--OSA--parts002.png|600px|thumb|center|8-17 DNAzyme(two parts) Pb2+ testing]] |
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− | ===Usage and Biology=== | + | ===Usage and Biology===--> |
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Revision as of 14:49, 25 October 2020
A type of DNA oligonucleotide that can cleave towards its substrate after binding to Pb2+.
The system is put into the enzyme marker and reacted for 60 minutes at 37℃. From the detected fluorescence, we determine that:
8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]