Difference between revisions of "Part:BBa K3657022"
Line 5: Line 5:
 
<html>
 
<html>
 
   
 
   
−
<p>It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (<a href= "https://parts.igem.org/Part:BBa_K3657023">BBa_K3657023</a>) in vitro.</p>
+
<p>It was proved by Knukler et al. that the transcript of this sequence creates a triple helix with the DNA part (<a href= "https://parts.igem.org/Part:BBa_K3657023">BBa_K3657023</a>) in vitro.</p>
 
<p>More information available here: LINK</p>
 
<p>More information available here: LINK</p>
  
Line 16: Line 16:
 
<ol>
 
<ol>
 
   <li>Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.</li>
 
   <li>Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.</li>
−
   <li>Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.</li>
+
   <li>Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knukler et al.</li>
 
</ol>
 
</ol>
 
</p>
 
</p>

Revision as of 14:48, 25 October 2020


RNA Part of an RNA·DNA-DNA triple helix

It was proved by Knukler et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.

More information available here: LINK

Usage and Biology

Triple helices are one of the alternative structures formed by nucleic acids. In addition to the Watson-Crick interactions between base pairs, Hoogsteen interactions are crucial for the formation of the triple helix. The binding is sequence specific.

Part Design

You can generate analogical parts yourself as following:

  1. Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.
  2. Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knukler et al.

References

Kunkler CN, Hulewicz JP, Hickman SC, Wang MC, McCown PJ, Brown JA. Stability of an RNA•DNA-DNA triple helix depends on base triplet composition and length of the RNA third strand. Nucleic Acids Res. 2019; 47(14):7213-7222.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]