Difference between revisions of "Part:BBa K3402060"
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<partinfo>BBa_K3402060 short</partinfo> | <partinfo>BBa_K3402060 short</partinfo> | ||
| − | This device is composed of P<i>tef1</i> (BBa_K3402007), <i>Cas9</i> (BBa_K3402023), <i>NLS</i> (BBa_K3402008) and T<i>syn7</i> (BBa_K3402001). | + | This device is composed of P<i>tef1</i> ([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), <i>Cas9</i> ([https://parts.igem.org/Part:BBa_K3402023# BBa_K3402023]), <i>NLS</i> ([https://parts.igem.org/Part:BBa_K3402008# BBa_K3402008]) and T<i>syn7</i> ([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]). |
[[Image:Cas9 expression cassette without homologous arms.png|400px]] | [[Image:Cas9 expression cassette without homologous arms.png|400px]] | ||
Latest revision as of 14:47, 25 October 2020
Cas9 expression cassette without homologous arms
This device is composed of Ptef1 (BBa_K3402007), Cas9 (BBa_K3402023), NLS (BBa_K3402008) and Tsyn7 (BBa_K3402001).
Usage and Biology
The use of the strongest promoter Ptef1 to express Cas9 protein and sgRNA could increase the gene-editing efficiency. As the Cas9 protein was too big to transport into the nucleus, a nuclear localization sequence (NLS) was needed to help the transportation of Cas9 protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5675
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1730
Illegal BglII site found at 5294
Illegal XhoI site found at 1051
Illegal XhoI site found at 1780
Illegal XhoI site found at 2143
Illegal XhoI site found at 5095 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5675
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5675
Illegal NgoMIV site found at 2557
Illegal NgoMIV site found at 3661 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1501
Illegal BsaI site found at 2218