Difference between revisions of "Part:BBa K3429002"
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The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. <sup> [1] </sup> We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB. | The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. <sup> [1] </sup> We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB. | ||
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| + | [1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2 | ||
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Revision as of 14:42, 25 October 2020
Superfolder Green Fluorescent Protein (sfGFP)
Superfolder Green Fluorescent Protein (sfGFP)
The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. [1] We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB.
References
[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]