Difference between revisions of "Part:BBa K3429002"
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| − | The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. We implement it as a reporter and experimental control for our TasA fusion proteins with CotA, CuO and EreB. | + | The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. <sup> [1] </sup> We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB. |
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Revision as of 14:38, 25 October 2020
Superfolder Green Fluorescent Protein (sfGFP)
Superfolder Green Fluorescent Protein (sfGFP)
The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. [1] We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB.
<a class="anchor" id="cite_note-1"></a>
<a class="referencestd" href="https://doi.org/10.1038/s41589-018-0169-2 " target="_blank">7. Huang, J., Liu, S., Zhang, C. et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41 (2019) </a>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]