Difference between revisions of "Part:BBa K3402058"
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<partinfo>BBa_K3402058 short</partinfo>
 
<partinfo>BBa_K3402058 short</partinfo>
  
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This device is composed of up-do<i>Leu</i> (BBa_K3402048), sg<i>Leu</i> (BBa_K3402049), 50bp-up<i>PXA1</i> (BBa_K3402037), <i>hph</i> (BBa_K3402012), 50bp-do<i>PXA1</i> (BBa_K3402038), P<i>tef1</i> (BBa_K3402007), sg<i>PXA1</i> (BBa_K3402039), sg<i>GFP</i> (BBa_K3402024), T<i>syn7</i> (BBa_K3402001), up<i>GFP</i> (BBa_K3402025), do<i>GFP</i> (BBa_K3402026).
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This device is composed of up-do<i>Leu</i> ([https://parts.igem.org/Part:BBa_K3402048# BBa_K3402048]), sg<i>Leu</i> ([https://parts.igem.org/Part:BBa_K3402049# BBa_K3402049]), 50bp-up<i>PXA1</i> ([https://parts.igem.org/Part:BBa_K3402037# BBa_K3402037]), <i>hph</i> ([https://parts.igem.org/Part:BBa_K3402012# BBa_K3402012]), 50bp-do<i>PXA1</i> ([https://parts.igem.org/Part:BBa_K3402038# BBa_K3402038]), P<i>tef1</i> ([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), sg<i>PXA1</i> ([https://parts.igem.org/Part:BBa_K3402039# BBa_K3402039]), sg<i>GFP</i> ([https://parts.igem.org/Part:BBa_K3402024# BBa_K3402024]), T<i>syn7</i> ([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]), up<i>GFP</i> ([https://parts.igem.org/Part:BBa_K3402025# BBa_K3402025]), do<i>GFP</i> ([https://parts.igem.org/Part:BBa_K3402026# BBa_K3402026]).
  
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[[Image:Triple-gene editing cassette.png|700px]]
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[[Image:Triple-gene editing cassette.png|600px]]
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 14:36, 25 October 2020


Triple-gene editing cassette

This device is composed of up-doLeu (BBa_K3402048), sgLeu (BBa_K3402049), 50bp-upPXA1 (BBa_K3402037), hph (BBa_K3402012), 50bp-doPXA1 (BBa_K3402038), Ptef1 (BBa_K3402007), sgPXA1 (BBa_K3402039), sgGFP (BBa_K3402024), Tsyn7 (BBa_K3402001), upGFP (BBa_K3402025), doGFP (BBa_K3402026).

Triple-gene editing cassette.png

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 776
    Illegal XhoI site found at 3223
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 174
    Illegal NgoMIV site found at 203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3859