Difference between revisions of "Part:BBa K3589109"

 
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This basic part contains the coding sequence of a wildtype laccase from an uncultured marine bacteria (B3-B4). Combined with a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 1-3 of RBCS2, it perfectly matches the part <a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a> (pAR promoter A1-B2), resulting in a high expression (<a href="https://www.researchgate.net/publication/23762345_Strategies_to_facilitate_transgene_expression_in_Chlamydomonas_reinhardtii">Eichler-Stahlberg et al., 2009</a>). To detect the target protein a 3xHA-tag from the Kaiser Collection (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) is recommended.
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We recommend using the pAR promoter (A1-A3) (<a href="https://parts.igem.org/Part:BBa_K3002003">BBa_K3002003</a>).
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This part was designed to allow synthesis due to a high GC-level.
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Revision as of 14:12, 25 October 2020


Wildtype laccase from uncultured marine bacteria for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of a wildtype laccase from an uncultured marine bacteria (B3-B4). Combined with a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 1-3 of RBCS2, it perfectly matches the part BBa_K3002027 (pAR promoter A1-B2), resulting in a high expression (Eichler-Stahlberg et al., 2009). To detect the target protein a 3xHA-tag from the Kaiser Collection (BBa_K3002017) is recommended. We recommend using the pAR promoter (A1-A3) (BBa_K3002003).
This part was designed to allow synthesis due to a high GC-level.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 817
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 817
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 1396
    Illegal NgoMIV site found at 1847
  • 1000
    COMPATIBLE WITH RFC[1000]