Difference between revisions of "Part:BBa K3589108"

 
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This basic part contains the coding sequence of the laccase from the ascomycete <I>Botrytis aclada</i> mutant  L499F (B3 – B4). This part is codon-optimized for <I>Chlamydomonas reinhardtii</I>. Combined with a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 1 – 3 of RBCS2, it perfectly matches the part <a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a> (pAR promoter A1-B2), resulting in a high expression (<a href="https://www.researchgate.net/publication/23762345_Strategies_to_facilitate_transgene_expression_in_Chlamydomonas_reinhardtii">Eichler-Stahlberg et al., 2009</a>).
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To detect the target protein a 3xHA-tag from the Kaiser Collection (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) is recommended.
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We recommend using the pAR promoter (A1-A3) (<a href="https://parts.igem.org/Part:BBa_K3002003">BBa_K3002003</a>).
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This part was designed to allow synthesis due to a high GC-level.
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Revision as of 13:56, 25 October 2020


Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of the laccase from the ascomycete Botrytis aclada mutant L499F (B3 – B4). This part is codon-optimized for Chlamydomonas reinhardtii. Combined with a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 1 – 3 of RBCS2, it perfectly matches the part BBa_K3002027 (pAR promoter A1-B2), resulting in a high expression (Eichler-Stahlberg et al., 2009). To detect the target protein a 3xHA-tag from the Kaiser Collection (BBa_K3002017) is recommended. We recommend using the pAR promoter (A1-A3) (BBa_K3002003).
This part was designed to allow synthesis due to a high GC-level.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
    Illegal PstI site found at 1496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
    Illegal PstI site found at 1496
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1055
    Illegal BglII site found at 2014
    Illegal BamHI site found at 1394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
    Illegal PstI site found at 1496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
    Illegal PstI site found at 1496
  • 1000
    COMPATIBLE WITH RFC[1000]