Difference between revisions of "Part:BBa K3380622"
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<partinfo>BBa_K3380622 short</partinfo> | <partinfo>BBa_K3380622 short</partinfo> | ||
| − | '''Figure 1''' illustrates a schematic design of the construct. | + | The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (Broccoli BBa_K3380153) under an ArsR transcription factor regulated promoter (BBa_K190015). As opposed to BBa_K3380604, this does not have a scaffold. It was designed to test the ArsR regulated promoter and to assess the impact of the tRNA scaffold absence on the RNA aptamer fluorescence. Simultaneously we tested the efficiency of transcription in a cell-free extract when adding a T7 terminator (BBa_K3137010) as compared to the "run off" transcription (BBa_K3380610) from a linear DNA with no terminator. The construct is capable of exhibiting fluorescence being a transcription only construct. The T7 RNA polymerase can synthesize transcripts via run-off transcription in the absence of a terminator (because the DNA template is linear). '''Figure 1''' illustrates a schematic design of the construct. |
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[[File:Construct 622.png|500px|]] | [[File:Construct 622.png|500px|]] | ||
Revision as of 13:35, 25 October 2020
No scaffold TXO ArsR regulated Broccoli construct under T7 P(BBa_K190015) and (BBa_K3137010) T
The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (Broccoli BBa_K3380153) under an ArsR transcription factor regulated promoter (BBa_K190015). As opposed to BBa_K3380604, this does not have a scaffold. It was designed to test the ArsR regulated promoter and to assess the impact of the tRNA scaffold absence on the RNA aptamer fluorescence. Simultaneously we tested the efficiency of transcription in a cell-free extract when adding a T7 terminator (BBa_K3137010) as compared to the "run off" transcription (BBa_K3380610) from a linear DNA with no terminator. The construct is capable of exhibiting fluorescence being a transcription only construct. The T7 RNA polymerase can synthesize transcripts via run-off transcription in the absence of a terminator (because the DNA template is linear). Figure 1 illustrates a schematic design of the construct.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]