Difference between revisions of "Part:BBa K3380618"
| Line 3: | Line 3: | ||
<partinfo>BBa_K3380618 short</partinfo> | <partinfo>BBa_K3380618 short</partinfo> | ||
| − | '''Figure 1''' illustrates a schematic design of the construct. | + | The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (iSpinach BBa_K3380150) under an ArsR transcription factor regulated promoter (BBa_K190015). As opposed to BBa_K3380603, this does not have a scaffold. It was designed to test the ArsR regulated promoter and to assess the impact of the tRNA scaffold absence on the RNA aptamer fluorescence. The construct is capable of exhibiting fluorescence being a transcription only construct. Simultaneously we tested the efficiency of transcription in the absence of a terminator in a cell-free extract. The T7 RNA polymerase is capable of synthesizing transcripts via run-off transcription in the absence of a terminator (because the DNA template is linear). '''Figure 1''' illustrates a schematic design of the construct. |
| − | [[File: | + | [[File:Construct618v1.png|500px|]] |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 13:11, 25 October 2020
No scaffold TXO ArsR regulated iSpinach construct under T7 P(BBa_K3380103) and (BBa_K3137010) T
The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (iSpinach BBa_K3380150) under an ArsR transcription factor regulated promoter (BBa_K190015). As opposed to BBa_K3380603, this does not have a scaffold. It was designed to test the ArsR regulated promoter and to assess the impact of the tRNA scaffold absence on the RNA aptamer fluorescence. The construct is capable of exhibiting fluorescence being a transcription only construct. Simultaneously we tested the efficiency of transcription in the absence of a terminator in a cell-free extract. The T7 RNA polymerase is capable of synthesizing transcripts via run-off transcription in the absence of a terminator (because the DNA template is linear). Figure 1 illustrates a schematic design of the construct.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 25