Difference between revisions of "Part:BBa K3589106"
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<partinfo>BBa_K3589106 short</partinfo> | <partinfo>BBa_K3589106 short</partinfo> | ||
− | This basic part contains the coding sequence of the | + | |
+ | This basic part contains the coding sequence of the wildtype form of a laccase from an uncultured marine bacteria (hereafter referred to as marLac). This part is codon-optimized for <I>Escherichia coli</I>. Combined a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. This part can be cloned into standard <I>E coli</I>. expression vectors like the commercially available pGEX-6P-1 vector.<br> | ||
+ | This laccase is cold-adapted, thermostable and shows a high tolerance for organic solvents as well as salt (Yang <I> et. al</I>, 2018; doi: 10.3389/fmicb.2018.02998). | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 13:03, 25 October 2020
Wildtype laccase from uncultured marine bacteria for Escherichia coli
This basic part contains the coding sequence of the wildtype form of a laccase from an uncultured marine bacteria (hereafter referred to as marLac). This part is codon-optimized for Escherichia coli. Combined a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. This part can be cloned into standard E coli. expression vectors like the commercially available pGEX-6P-1 vector.
This laccase is cold-adapted, thermostable and shows a high tolerance for organic solvents as well as salt (Yang et. al, 2018; doi: 10.3389/fmicb.2018.02998).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 517
Illegal PstI site found at 22
Illegal PstI site found at 52 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 517
Illegal PstI site found at 22
Illegal PstI site found at 52 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 517
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 517
Illegal PstI site found at 22
Illegal PstI site found at 52 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 517
Illegal PstI site found at 22
Illegal PstI site found at 52
Illegal NgoMIV site found at 840
Illegal AgeI site found at 93
Illegal AgeI site found at 1105 - 1000COMPATIBLE WITH RFC[1000]