Difference between revisions of "Part:BBa K3373014"

Latest revision as of 12:10, 25 October 2020

BSR_NucA-TEVsite_UVR8-NtTEV_COP1-CtTEV

This part is a multigenic construct containing our three constructs for the operation of our kill-switch system adapted to C. reinhardtii. The CDS of the Nuclease A (NucA) fused to a double nuclear localization signal SV40 NLS (BBa_K3373008) is associated at the N-terminus with the HAP2 transmembrane domain (BBa_K3373007) and at the C-terminus with the recognition site for the TEV protease associated with a peptide linker (BBa_K3373011). The CDS of UVR8 (BBa_K3373005) is fused to the C-terminus domain of the TEV protease (BBa_K3373010) with the GSAT peptide linker (BBa_K3373006). The CDS of COP1 (BBa_K3373004) is fused to the N-terminus domain of the TEV protease (BBa_K3373009) with the GSAT peptide linker (BBa_K3373006). All these CDS are under the control of the PSAD promoter and terminator which allow a strong expression in C. reinhardtii. It also contains a Blasticidin resistance cassette (BSR) encoding for the Blasticidin S Deaminase in order to select the recombinant clones after transformation in C. reinhardtii.

Sequence and Features