Difference between revisions of "Part:BBa K3468070:Experience"

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===Applications of BBa_K3468070===
 
===Applications of BBa_K3468070===
 
The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase.
 
The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase.
[[File:T260R.png|400px|thumb|left|Fig.1 Thr266 in PyMOL]]
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[[File:T270Q.png|400px|thumb|left|Fig.1 T270Q in PyMOL]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 09:42, 25 October 2020


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Please enter how you used this part and how it worked out.

Applications of BBa_K3468070

The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase.

Fig.1 T270Q in PyMOL

User Reviews

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