Difference between revisions of "Part:BBa K3468046:Experience"
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===Applications of BBa_K3468046=== | ===Applications of BBa_K3468046=== | ||
| + | The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bond with 143-SER. after it was mutated to ARG, it formed hydrogen bond with 138-ASN, which stabilized α3 helix and α3-β5loop, which was beneficial to improve the thermal stability of PETase. | ||
| + | [[File:Y146R.png|400px|thumb|left|Fig.1 Y146R in PyMOL]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 09:35, 25 October 2020
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Applications of BBa_K3468046
The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bond with 143-SER. after it was mutated to ARG, it formed hydrogen bond with 138-ASN, which stabilized α3 helix and α3-β5loop, which was beneficial to improve the thermal stability of PETase.
File:Y146R.png
Fig.1 Y146R in PyMOL
User Reviews
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