Difference between revisions of "Part:BBa K3468045:Experience"

 
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===Applications of BBa_K3468045===
 
===Applications of BBa_K3468045===
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The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bond with 51-THR. after mutation to ARG, it formed hydrogen bond with 73-ASN and 148-LYS, which stabilized α2-β3loop and α3-β5loop, which was beneficial to improve the thermal stability of PETase.
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[[File:T72R.png|400px|thumb|left|Fig.1 T72R in PyMOL]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 09:33, 25 October 2020


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Please enter how you used this part and how it worked out.

Applications of BBa_K3468045

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bond with 51-THR. after mutation to ARG, it formed hydrogen bond with 73-ASN and 148-LYS, which stabilized α2-β3loop and α3-β5loop, which was beneficial to improve the thermal stability of PETase.

Fig.1 T72R in PyMOL

User Reviews

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