Difference between revisions of "Part:BBa K3468040:Experience"
| Line 5: | Line 5: | ||
===Applications of BBa_K3468040=== | ===Applications of BBa_K3468040=== | ||
| + | It was predicted by FoldX that DDG decreased. PyOML was used for visual screening, which formed hydrogen bonds with 78-VAL, 144-Pro and 145-ILE. after mutation to ARG, it formed new hydrogen bonds with 71-PRO and 72-THR, which stabilized α3-β5loop, β2-β3loop and β2sheet, | ||
| + | which was beneficial to improve the thermal stability of PETase | ||
| + | [[File:K148R.png|400px|thumb|left|Fig.1 K148R in PyMOL]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 09:17, 25 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3468040
It was predicted by FoldX that DDG decreased. PyOML was used for visual screening, which formed hydrogen bonds with 78-VAL, 144-Pro and 145-ILE. after mutation to ARG, it formed new hydrogen bonds with 71-PRO and 72-THR, which stabilized α3-β5loop, β2-β3loop and β2sheet,
which was beneficial to improve the thermal stability of PETase
User Reviews
UNIQ589ce656464499a4-partinfo-00000000-QINU UNIQ589ce656464499a4-partinfo-00000001-QINU