Difference between revisions of "Part:BBa K3606025"

 
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<partinfo>BBa_K3606025 short</partinfo>
 
<partinfo>BBa_K3606025 short</partinfo>
  
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<h2>Background:</h2>
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<h2>Design:</h2>
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This part is a composite part of PtetR[[BBa_R0040]] and mcbEFG[[BBa_K3606004]].
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<h2>Usage and Biology:</h2>
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PtetR determines the expression of mcbEFG when the number of bacteria is at different level.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3606025 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3606025 SequenceAndFeatures</partinfo>
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<h2>Further Application:</h2>
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For futher application, this part is provided as an immunity system for antimicrobial peptide(mccb17). This part can be used together with [[BBa_K3606003]] to express antimicrobial peptide(mccb17) and create survival advantage for the engineered strain. These part are especially useful to be expressed in vivo because research has proved that it can also ease the inflammation in intestine by limiting the expansion of related pathogens and pathobionts.
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<h2>References:</h2>
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Collin F, Maxwell A. The Microbial Toxin Microcin B17: Prospects for the Development of New Antibacterial Agents. J Mol Biol. 2019;431(18):3400–3426. doi:10.1016/j.jmb.2019.05.050
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S. Duquesne, D. Destoumieux-Garzón, J. Peduzzi, S. Rebuffat. Microcins, gene-encoded antibacterial peptides from enterobacteria
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Sassone-Corsi M, Nuccio SP, Liu H, Hernandez D, Vu CT, Takahashi AA, Edwards RA, Raffatellu M. Microcins mediate competition among Enterobacteriaceae in the inflamed gut. Nature. 2016 Dec 8;540(7632):280-283.
  
  

Revision as of 09:09, 25 October 2020


PtetR driven mcbEFG

Background:


Design:

This part is a composite part of PtetRBBa_R0040 and mcbEFGBBa_K3606004.

Usage and Biology:

PtetR determines the expression of mcbEFG when the number of bacteria is at different level.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Further Application:

For futher application, this part is provided as an immunity system for antimicrobial peptide(mccb17). This part can be used together with BBa_K3606003 to express antimicrobial peptide(mccb17) and create survival advantage for the engineered strain. These part are especially useful to be expressed in vivo because research has proved that it can also ease the inflammation in intestine by limiting the expansion of related pathogens and pathobionts.

References:

Collin F, Maxwell A. The Microbial Toxin Microcin B17: Prospects for the Development of New Antibacterial Agents. J Mol Biol. 2019;431(18):3400–3426. doi:10.1016/j.jmb.2019.05.050 S. Duquesne, D. Destoumieux-Garzón, J. Peduzzi, S. Rebuffat. Microcins, gene-encoded antibacterial peptides from enterobacteria Sassone-Corsi M, Nuccio SP, Liu H, Hernandez D, Vu CT, Takahashi AA, Edwards RA, Raffatellu M. Microcins mediate competition among Enterobacteriaceae in the inflamed gut. Nature. 2016 Dec 8;540(7632):280-283.