Difference between revisions of "Part:BBa K3468037:Experience"
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===Applications of BBa_K3468037===
 
===Applications of BBa_K3468037===
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The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.
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The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.
 
[[File:Ala152.png|400px|thumb|left|Fig.1 Ala152 in PyMOL]]
 
[[File:Ala152.png|400px|thumb|left|Fig.1 Ala152 in PyMOL]]
 
[[File:A152N.png|400px|thumb|left|Fig.2 A152N in PyMOL]]
 
[[File:A152N.png|400px|thumb|left|Fig.2 A152N in PyMOL]]

Revision as of 09:03, 25 October 2020


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Applications of BBa_K3468037

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.

Fig.1 Ala152 in PyMOL
Fig.2 A152N in PyMOL

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