Difference between revisions of "Part:BBa K3468037:Experience"
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===Applications of BBa_K3468037=== | ===Applications of BBa_K3468037=== | ||
| + | The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase. | ||
| + | [[File:Ala152.png|400px|thumb|left|Fig.1 Ala152 in PyMOL]] | ||
| + | [[File:A152N.png|400px|thumb|left|Fig.2 A152N in PyMOL]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 09:03, 25 October 2020
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Applications of BBa_K3468037
The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.
User Reviews
UNIQc69db58ad349a2b5-partinfo-00000000-QINU UNIQc69db58ad349a2b5-partinfo-00000001-QINU