Difference between revisions of "Part:BBa K3606007:Design"
 
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===Design Notes===
 
===Design Notes===
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This part functions to lower the leakage and raise the level of expression of the downstream gene. As a well-characterized promoter, ptetR has been studied thoroughly with its expression level and leakage. While here we take a unique idea to created a new promoter fusion: we fuse the -35 upstream, -35--10 parts of the stronger pl promoters in lambda phages with the tetO operon regulated by tetracycline. As a promoter fusion of pl and tetO, not only is it regulated by tetR and anhydrotetracycline, but it also inherited the expression level of pl.  
+
This part functions to lower the leakage and raise the level of expression of the downstream gene. As a well-characterized promoter, PtetR has been studied thoroughly with its expression level and leakage. While here we take a unique idea to created a new promoter fusion: we fuse the -35 upstream, -35--10 parts of the stronger PL promoters in lambda phages with the tetO operon regulated by tetracycline. As a promoter fusion of PL and tetO, not only is it regulated by tetR and anhydrotetracycline, but it also inherited the expression level of PL.  
  
  
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===References===
 
===References===
  
−
Yingna Qiu, et al. The construction of rigorous type promoter,i on E. coli chromosomes. Microbiology China,2018,45(08):1693-1704.
+
Yingna Qiu, et al. The construction of rigorous type promoter on E. coli chromosomes. Microbiology China,2018,45(08):1693-1704.

Latest revision as of 08:58, 25 October 2020


PtetO3 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part functions to lower the leakage and raise the level of expression of the downstream gene. As a well-characterized promoter, PtetR has been studied thoroughly with its expression level and leakage. While here we take a unique idea to created a new promoter fusion: we fuse the -35 upstream, -35--10 parts of the stronger PL promoters in lambda phages with the tetO operon regulated by tetracycline. As a promoter fusion of PL and tetO, not only is it regulated by tetR and anhydrotetracycline, but it also inherited the expression level of PL.


Source

whole sequence synthesized

References

Yingna Qiu, et al. The construction of rigorous type promoter on E. coli chromosomes. Microbiology China,2018,45(08):1693-1704.