Difference between revisions of "Part:BBa K3468071:Experience"

 
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===Applications of BBa_K3468071===
 
===Applications of BBa_K3468071===
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According to the prediction of FoldX, DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into SER, it formed hydrogen bonds with GLY-48 and SER-103, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase.
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[[File:Ala47.png|400px|thumb|left|Fig.1 Ala47 in PyMOL]]
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[[File:A47S.png|400px|thumb|left|Fig.2 A47S in PyMOL]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 08:47, 25 October 2020


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3468071

According to the prediction of FoldX, DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into SER, it formed hydrogen bonds with GLY-48 and SER-103, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase.

Fig.1 Ala47 in PyMOL
Fig.2 A47S in PyMOL

User Reviews

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