Difference between revisions of "Part:BBa K3606033:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We linked DsbA to the N-terminal of CAAP for its secretion into the extracellular space.
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<p> The composite part DsbA_CaAP is a combination of signal peptide DsbA and CaAP(Calcium Absorption Peptide). The sequence of DsbA is obtained from BBa_K1659002 with no further codon optimization. Then we fused DsbA(BBa_K3606043) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space. </p>
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Latest revision as of 08:41, 25 October 2020


DsbA-CaAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part DsbA_CaAP is a combination of signal peptide DsbA and CaAP(Calcium Absorption Peptide). The sequence of DsbA is obtained from BBa_K1659002 with no further codon optimization. Then we fused DsbA(BBa_K3606043) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space.


Source

The sequence of DsbA is obtained from BBa_K1659002 with no further codon optimization while the sequence of CAAP is artificially designed based on oligopeptides obtained from tilapia bone collagen hydrolysate, etc.

References