Difference between revisions of "Part:BBa K150003"

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Chloramphenicol resistance cassette including promotor, RBS, coding sequence and terminator.<br>
 
Chloramphenicol resistance cassette including promotor, RBS, coding sequence and terminator.<br>
In a short characterization it was shown that this part leads to acceptable resistance up to 100 µg/ml, even in 500 µg/ml chloramphenicol unmistakably cell growth can be detected. The effect of the solvent ethanol can even be at high concentrations neglected.
+
In a short characterization it was shown that this part leads to acceptable resistance up to 100 µg/ml chloramphenicol, even in 500 µg/ml unmistakably cell growth can be detected. The effect of the solvent ethanol can even be at high concentrations neglected.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:59, 29 October 2008

Chloramphenicol resistance cassette

Chloramphenicol resistance cassette including promotor, RBS, coding sequence and terminator.
In a short characterization it was shown that this part leads to acceptable resistance up to 100 µg/ml chloramphenicol, even in 500 µg/ml unmistakably cell growth can be detected. The effect of the solvent ethanol can even be at high concentrations neglected.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]