Difference between revisions of "Part:BBa K3606031:Design"
Zhenru-Zhang (Talk | contribs) |
Zhenru-Zhang (Talk | contribs) |
||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | <p> | + | <p> The composite part Nsp4_CaAP is a combination of signal peptide Nsp4 and CaAP(Calcium Absorption Peptide). The sequence of Nsp4 is obtained from BBa_K3021004 with no further codon optimization. Then we fused Nsp4(BBa_K3606042) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space. </p> |
− | + | ||
===Source=== | ===Source=== |
Latest revision as of 08:37, 25 October 2020
Nsp4-CaAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
Illegal AgeI site found at 135 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The composite part Nsp4_CaAP is a combination of signal peptide Nsp4 and CaAP(Calcium Absorption Peptide). The sequence of Nsp4 is obtained from BBa_K3021004 with no further codon optimization. Then we fused Nsp4(BBa_K3606042) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space.
Source
The sequences of subparts are obtained from E.coli. genome.