Difference between revisions of "Part:BBa K150003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K150003 short</partinfo> | <partinfo>BBa_K150003 short</partinfo> | ||
− | Chloramphenicol resistance cassette | + | Chloramphenicol resistance cassette including promotor, RBS, coding sequence and terminator.<br> |
+ | In a short characterization it was shown that this part leads to acceptable resistance up to 100 µg/ml, even in 500 µg/ml chloramphenicol unmistakably cell growth can be detected. The effect of the solvent ethanol can even be at high concentrations neglected. | ||
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Revision as of 16:58, 29 October 2008
Chloramphenicol resistance cassette
Chloramphenicol resistance cassette including promotor, RBS, coding sequence and terminator.
In a short characterization it was shown that this part leads to acceptable resistance up to 100 µg/ml, even in 500 µg/ml chloramphenicol unmistakably cell growth can be detected. The effect of the solvent ethanol can even be at high concentrations neglected.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]