Difference between revisions of "Part:BBa K3440001"

Revision as of 08:25, 25 October 2020

mCherry under bphR1 promoter

PbphR1(BBa_K1155001)-RBS(BBa_B0034)-mCherry(BBa_J06504)-DT(BBa_B0015)

Usage and Biology

Produces mCherry under bphR1 promoter, which can be activated by bphR2:Polychlorinated biphenyl.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.

Colony PCR gel for BBa_K34440001 (noted B)

Fluorescence measurements for BBa_K34440001 (C) and BBa_K3440002 (B)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 156
Illegal BamHI site found at 239
Illegal XhoI site found at 46
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 139

@@ Line 12: / Line 12: @@
 We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.
-[[File:T--Stockholm--BBa_K34440001_and_BBa_K3440002_WB.png|thumb|center|500px|Colony PCR Gel for BBa_K34440001 (C) and BBa_K3440003 (D)]]
+[[File:T--Stockholm--GelB.png|thumb|center|500px|Colony PCR gel for BBa_K34440001 (noted B)]]
 [[File:T--Stockholm--BBa_K34440001_and_BBa_K3440002_Fluo.png|thumb|center|1000px|Fluorescence measurements for BBa_K34440001 (C) and BBa_K3440002 (B)]]