Difference between revisions of "Part:BBa K3365007"
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<partinfo>BBa_K3365007 short</partinfo>
 
<partinfo>BBa_K3365007 short</partinfo>
  
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The PAM and potential off-target sequence is located directly downstream of the promoter, where dCas9 might bind wrongly and block RNAP. In our part, the “Lure1” is the potential off-target sequence for PDCD1 CRISPR gene editing mentioned in one literature, which might be identified and bound by the complex of dCas9 and sgRNA.
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The PAM and potential off-target sequence is located directly downstream of the promoter, where dCas9 might bind wrongly and block RNAP. In our part, the “lure1” is the potential off-target sequence for PDCD1 CRISPR gene editing mentioned in one literature, which might be identified and bound by the complex of dCas9 and sgRNA.
  
 
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Revision as of 08:23, 25 October 2020


Lure1 sequence downstream of pBAD

The PAM and potential off-target sequence is located directly downstream of the promoter, where dCas9 might bind wrongly and block RNAP. In our part, the “lure1” is the potential off-target sequence for PDCD1 CRISPR gene editing mentioned in one literature, which might be identified and bound by the complex of dCas9 and sgRNA.

Usage and Biology

The uninduced transcriptional level downstream the signaling is very low. In the presence of arabinose, transcription from the pBAD promoter is turned on. In the presence of both arabinose and the complex of dCas9 and sgRNA, the transcription might be partially inhibited because of the potential block of RNAP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56