Difference between revisions of "Part:BBa K3606031:Design"

 
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===Design Notes===
 
===Design Notes===
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We linked NSP4 to the N-terminal of CAAP for its secretion into the extracellular space.
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<p>Part BBa_K3606031 is a new composite part designed to allow CAAP secretion from our engineered E.coli strain by adding a signal peptide novel signal peptide 4 (Nsp4) to the N terminus of CAAP. As a functional peptide that allows surface anchoring of its fused gene product, Nsp4 enables CAAP to be presented on the cellular surface of E.coli. </p>
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Revision as of 08:12, 25 October 2020


Nsp4-CaAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
    Illegal AgeI site found at 135
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part BBa_K3606031 is a new composite part designed to allow CAAP secretion from our engineered E.coli strain by adding a signal peptide novel signal peptide 4 (Nsp4) to the N terminus of CAAP. As a functional peptide that allows surface anchoring of its fused gene product, Nsp4 enables CAAP to be presented on the cellular surface of E.coli.


Source

The sequences of subparts are obtained from E.coli. genome.

References