Difference between revisions of "Part:BBa K3606914"

 
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<partinfo>BBa_K3606914 short</partinfo>
 
<partinfo>BBa_K3606914 short</partinfo>
  
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tba
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BBa_K3606104 is one of a series of composite parts designed to screen a suitable strength promoter for mcbABCD.
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<h2>Usage and Biology:</h2>
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P14, which is a constitutive promoter, will continuously drive expression of mcbABCD. McbABCD is responsible for producing MccB17.
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<h2>Design:</h2>
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In our design the proper expression level of mcbABCD is very important. Since its product MccB17 acts as antibiotic, its low expression will make it unable to kill the surrounding bacteria and cannot improve its competitiveness in the intestine. But considering its toxicity, its high expression will also increase the burden of survival of engineered bacteria.
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So we need to test a collection of promoters, and find the most suitable one for it.
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<h2> Characterization:</h2>
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 +
 
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<h2>Further Application:</h2>
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To use this part, simply clone it into a low-copy plasmid vector, transfer into E.coli, incubate overnight. You can also understand the strength of P14 through my experimental results.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:48, 25 October 2020


P14 driven mcbABCD

BBa_K3606104 is one of a series of composite parts designed to screen a suitable strength promoter for mcbABCD.

Usage and Biology:

P14, which is a constitutive promoter, will continuously drive expression of mcbABCD. McbABCD is responsible for producing MccB17.

Design:

In our design the proper expression level of mcbABCD is very important. Since its product MccB17 acts as antibiotic, its low expression will make it unable to kill the surrounding bacteria and cannot improve its competitiveness in the intestine. But considering its toxicity, its high expression will also increase the burden of survival of engineered bacteria. So we need to test a collection of promoters, and find the most suitable one for it.

Characterization:


Further Application:

To use this part, simply clone it into a low-copy plasmid vector, transfer into E.coli, incubate overnight. You can also understand the strength of P14 through my experimental results.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
    Illegal NgoMIV site found at 1989
    Illegal AgeI site found at 2161
  • 1000
    COMPATIBLE WITH RFC[1000]