Difference between revisions of "Part:BBa K3468064:Experience"

Revision as of 06:52, 25 October 2020

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Applications of BBa_K3468064

The thermal stability improvement result was obtained by the evaluation of I-Mutant website, and the thermal stability improvement result of -1.2251 was also obtained by the FoldX evaluation.

Fig.2 R260F in PyMOL

Fig.3 the result of R260F in I-Mutant

References

[1]Burley SK, Petsko GA. Aromatic-aromatic interaction: a mechanism of protein structure stabilization. Science. 1985 Jul 5;229(4708):23-8.

[2]Chatterjee KS, Tripathi V, Das R. A conserved and buried edge-to-face aromatic interaction in small ubiquitin-like modifier (SUMO) has a role in SUMO stability and function. J Biol Chem. 2019 Apr 26;294(17):6772-6784.

[3]Chrtis C, Jaffe R, Maigret B et al. , 1996. Benzene Dimer: A good model for π-π interactions in proteins a comparison between the benzene and the toluene dimers in the gas phase and in an aqueous solution [J] J. Am. Chem. Soc, 118: 11217-11224.

[4]Weber DS, Warren JJ. A survey of methionine-aromatic interaction geometries in the oxidoreductase class of enzymes: What could Met-aromatic interactions be doing near metal sites? J Inorg Biochem. 2018 Sep;186:34-41.

User Reviews

UNIQ239f186729bd8cb2-partinfo-00000000-QINU UNIQ239f186729bd8cb2-partinfo-00000001-QINU

@@ Line 7: / Line 7: @@
 The thermal stability improvement result was obtained by the evaluation of I-Mutant website, and the thermal stability improvement result of -1.2251 was also obtained by the FoldX evaluation.
-[[File:R260F222.png|400px|thumb|left|Fig.1 R260F in PyMOL]]
+[[File:R260F222.png|400px|thumb|left|Fig.2 R260F in PyMOL]]
-[[File:R260F.png|400px|thumb|left|Fig.1 the result of R260F in I-Mutant]]
+[[File:R260F.png|400px|thumb|left|Fig.3 the result of R260F in I-Mutant]]
 References