Difference between revisions of "Part:BBa K3490020:Design"

 
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===References===
 
===References===
 +
<br>[1]Guglielmini J, Van Melderen L. Bacterial toxin-antitoxin systems. Mobile Genetic Elements. 2011;1(4):283-306.
 +
<br>[2]Jørgensen MG, Pandey DP, Jaskolska M, Gerdes K. HicA of Escherichia coli Defines a Novel Family of Translation-Independent mRNA Interferases in Bacteria and Archaea. Journal of Bacteriology. 2008;191(4):1191-1199.‌
 +
<br>[3]Motta-Mena LB, Reade A, Mallory MJ, et al. An optogenetic gene expression system with rapid activation and deactivation kinetics. Nature Chemical Biology. 2014;10(3):196-202.
 +
<br>[4]Branda CS, Dymecki SM. Talking about a Revolution. Developmental Cell. 2004;6(1):7-28.
 +
<br>[5]Jechlinger W, Szostak MP, Witte A, Lubitz W. Altered temperature induction sensitivity of the lambda pR/cI857 system for controlled gene expression in Escherichia coli. FEMS Microbiology Letters. 1999;173(2):347-352.
 +
<br>[6]Ringrose L. Quantitative comparison of DNA looping in vitro and in vivo: chromatin increases effective DNA flexibility at short distances. The EMBO Journal. 1999;18(23):6630-6641.
 +
<br>[7]Park Y-N, Masison D, Eisenberg E, Greene LE. Application of the FLP/FRT system for conditional gene deletion in yeast Saccharomyces cerevisiae. Yeast. 2011;28(9):673-681.

Revision as of 05:38, 25 October 2020


Regulating the growth rate of bacteria.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1512
    Illegal SpeI site found at 1830
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1512
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2789
    Illegal SpeI site found at 1830
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1512
    Illegal BamHI site found at 2729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1512
    Illegal SpeI site found at 1830
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1512
    Illegal SpeI site found at 1830
    Illegal NgoMIV site found at 142
    Illegal AgeI site found at 367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2225
    Illegal BsaI.rc site found at 2265


Design Notes

This year, we're unable to transform EL222 into bacteria. Hence, we decide to use CI857 to replace EL222.


Source

Derived from IDT synthesis.

References


[1]Guglielmini J, Van Melderen L. Bacterial toxin-antitoxin systems. Mobile Genetic Elements. 2011;1(4):283-306.
[2]Jørgensen MG, Pandey DP, Jaskolska M, Gerdes K. HicA of Escherichia coli Defines a Novel Family of Translation-Independent mRNA Interferases in Bacteria and Archaea. Journal of Bacteriology. 2008;191(4):1191-1199.‌
[3]Motta-Mena LB, Reade A, Mallory MJ, et al. An optogenetic gene expression system with rapid activation and deactivation kinetics. Nature Chemical Biology. 2014;10(3):196-202.
[4]Branda CS, Dymecki SM. Talking about a Revolution. Developmental Cell. 2004;6(1):7-28.
[5]Jechlinger W, Szostak MP, Witte A, Lubitz W. Altered temperature induction sensitivity of the lambda pR/cI857 system for controlled gene expression in Escherichia coli. FEMS Microbiology Letters. 1999;173(2):347-352.
[6]Ringrose L. Quantitative comparison of DNA looping in vitro and in vivo: chromatin increases effective DNA flexibility at short distances. The EMBO Journal. 1999;18(23):6630-6641.
[7]Park Y-N, Masison D, Eisenberg E, Greene LE. Application of the FLP/FRT system for conditional gene deletion in yeast Saccharomyces cerevisiae. Yeast. 2011;28(9):673-681.