Difference between revisions of "Part:BBa K3596055"

Revision as of 04:54, 25 October 2020

PpdD Signal+Pilin gene

BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)^[1]. This part codes for the monomer of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires(e-PNs) along with the E.coli PpdD signal sequence to facilitate the construction of e-PNs at N terminal.

Characterization

The e-PN can be successfully expressed by express this part under the control of pTac with typeIV pilin assembly system, and the result was verified by Western Blot(figure 1).

Figure 1: Western Blot of the cell lysis of BIOT and BIOT-His(express e-PN and e-PN-His respectively); Antibody: Anti-His tag; e-PN-His: 7.7 kDa.

We made a protein film following the instructions from the paper we researched.^[1] And we eventually succeeded in producing stable voltage at about 0.35V with a current of 500nA or so.(figure 2).

Figure 2: The voltage and current of e-PN

References

[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.

Sequence and Features